cellgro dc medium Search Results


cellgro dc medium  (Cellgro)
86
Cellgro cellgro dc medium
Cellgro Dc Medium, supplied by Cellgro, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cellgro+dc+medium/15+vivo+x/10__1007_slash_978___1___4939___3625___0-2225-2-2
Average 86 stars, based on 1 article reviews
cellgro dc medium - by Bioz Stars, 2026-10
86/100 stars
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modc differentiation medium  (Miltenyi Biotec)
95
Miltenyi Biotec modc differentiation medium
FIGURE 2 | Expression of ANKRD55 and neighboring genes in immature <t>moDC.</t> (A–D) Monocytes were cultivated for 6 days <t>in</t> <t>IL-4/GM-CSF</t> medium for differentiation into immature moDC in the absence (colored curves) or presence (gray curves) of AM-580, and expression levels of the indicated genes were measured by qPCR at the start of the cultivation (day 0) and on days 1, 2, 4, and 6. Mean ± SEM of 3 independent measurements; Friedman test (followed by Dunn’s multiple comparison test) for comparison of data points in each curve with day 0 and Wilcoxon test for comparison of both curves (Control vs. AM580). (E–H) Effect of tolerogenic compounds on gene expression in immature moDC by qPCR. Concentrations of compounds are provided in the Materials and Methods. Mean ± SEM of maximum 5 independent measurements; Friedman test (followed by Dunn’s multiple comparison test). (I) Analysis of ALDH1A2, RARa, and RARg expression by qPCR. Mean ± SEM; n = 6; Friedman test (followed by Dunn’s multiple comparison test). (J) Expression levels of three discrete ANKRD55 splice variants 201, 202, and 204 in immature moDC. Mean ± SEM; n = 10; Wilcoxon test. (K) Classical (Cla; CD14hi/CD16-), intermediate (Int; CD14hi/CD16+), and non- classical (NC; CD14+/CD16+) monocytes were isolated and separately cultivated for 6 days to differentiate into moDC. Gene expression levels were measured in the original monocyte subsets (M) and derived moDC (D). Mean ± SEM; n = 3; Mann–Whitney test for comparison between monocytes and moDC for each gene and subset. *p ≤0.05, **p ≤0.01.
Modc Differentiation Medium, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cellgro+dc+medium/Mo-DC+Differentiation+Medium%2C+human/pm35111166-88-27-32
Average 95 stars, based on 1 article reviews
modc differentiation medium - by Bioz Stars, 2026-10
95/100 stars
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rpmi 1640 culture medium  (Cellgro)
86
Cellgro rpmi 1640 culture medium
FIGURE 2 | Expression of ANKRD55 and neighboring genes in immature <t>moDC.</t> (A–D) Monocytes were cultivated for 6 days <t>in</t> <t>IL-4/GM-CSF</t> medium for differentiation into immature moDC in the absence (colored curves) or presence (gray curves) of AM-580, and expression levels of the indicated genes were measured by qPCR at the start of the cultivation (day 0) and on days 1, 2, 4, and 6. Mean ± SEM of 3 independent measurements; Friedman test (followed by Dunn’s multiple comparison test) for comparison of data points in each curve with day 0 and Wilcoxon test for comparison of both curves (Control vs. AM580). (E–H) Effect of tolerogenic compounds on gene expression in immature moDC by qPCR. Concentrations of compounds are provided in the Materials and Methods. Mean ± SEM of maximum 5 independent measurements; Friedman test (followed by Dunn’s multiple comparison test). (I) Analysis of ALDH1A2, RARa, and RARg expression by qPCR. Mean ± SEM; n = 6; Friedman test (followed by Dunn’s multiple comparison test). (J) Expression levels of three discrete ANKRD55 splice variants 201, 202, and 204 in immature moDC. Mean ± SEM; n = 10; Wilcoxon test. (K) Classical (Cla; CD14hi/CD16-), intermediate (Int; CD14hi/CD16+), and non- classical (NC; CD14+/CD16+) monocytes were isolated and separately cultivated for 6 days to differentiate into moDC. Gene expression levels were measured in the original monocyte subsets (M) and derived moDC (D). Mean ± SEM; n = 3; Mann–Whitney test for comparison between monocytes and moDC for each gene and subset. *p ≤0.05, **p ≤0.01.
Rpmi 1640 Culture Medium, supplied by Cellgro, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cellgro+dc+medium/1640+rpmi/pm18708593-67-28-31
Average 86 stars, based on 1 article reviews
rpmi 1640 culture medium - by Bioz Stars, 2026-10
86/100 stars
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dc medium  (Cellgenix)
90
Cellgenix dc medium
FIGURE 2 | Expression of ANKRD55 and neighboring genes in immature <t>moDC.</t> (A–D) Monocytes were cultivated for 6 days <t>in</t> <t>IL-4/GM-CSF</t> medium for differentiation into immature moDC in the absence (colored curves) or presence (gray curves) of AM-580, and expression levels of the indicated genes were measured by qPCR at the start of the cultivation (day 0) and on days 1, 2, 4, and 6. Mean ± SEM of 3 independent measurements; Friedman test (followed by Dunn’s multiple comparison test) for comparison of data points in each curve with day 0 and Wilcoxon test for comparison of both curves (Control vs. AM580). (E–H) Effect of tolerogenic compounds on gene expression in immature moDC by qPCR. Concentrations of compounds are provided in the Materials and Methods. Mean ± SEM of maximum 5 independent measurements; Friedman test (followed by Dunn’s multiple comparison test). (I) Analysis of ALDH1A2, RARa, and RARg expression by qPCR. Mean ± SEM; n = 6; Friedman test (followed by Dunn’s multiple comparison test). (J) Expression levels of three discrete ANKRD55 splice variants 201, 202, and 204 in immature moDC. Mean ± SEM; n = 10; Wilcoxon test. (K) Classical (Cla; CD14hi/CD16-), intermediate (Int; CD14hi/CD16+), and non- classical (NC; CD14+/CD16+) monocytes were isolated and separately cultivated for 6 days to differentiate into moDC. Gene expression levels were measured in the original monocyte subsets (M) and derived moDC (D). Mean ± SEM; n = 3; Mann–Whitney test for comparison between monocytes and moDC for each gene and subset. *p ≤0.05, **p ≤0.01.
Dc Medium, supplied by Cellgenix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cellgro+dc+medium/dc+medium/pmc05331043-69-18-20
Average 90 stars, based on 1 article reviews
dc medium - by Bioz Stars, 2026-10
90/100 stars
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dulbecco s modified eagle s medium  (Cellgro)
86
Cellgro dulbecco s modified eagle s medium
FIGURE 2 | Expression of ANKRD55 and neighboring genes in immature <t>moDC.</t> (A–D) Monocytes were cultivated for 6 days <t>in</t> <t>IL-4/GM-CSF</t> medium for differentiation into immature moDC in the absence (colored curves) or presence (gray curves) of AM-580, and expression levels of the indicated genes were measured by qPCR at the start of the cultivation (day 0) and on days 1, 2, 4, and 6. Mean ± SEM of 3 independent measurements; Friedman test (followed by Dunn’s multiple comparison test) for comparison of data points in each curve with day 0 and Wilcoxon test for comparison of both curves (Control vs. AM580). (E–H) Effect of tolerogenic compounds on gene expression in immature moDC by qPCR. Concentrations of compounds are provided in the Materials and Methods. Mean ± SEM of maximum 5 independent measurements; Friedman test (followed by Dunn’s multiple comparison test). (I) Analysis of ALDH1A2, RARa, and RARg expression by qPCR. Mean ± SEM; n = 6; Friedman test (followed by Dunn’s multiple comparison test). (J) Expression levels of three discrete ANKRD55 splice variants 201, 202, and 204 in immature moDC. Mean ± SEM; n = 10; Wilcoxon test. (K) Classical (Cla; CD14hi/CD16-), intermediate (Int; CD14hi/CD16+), and non- classical (NC; CD14+/CD16+) monocytes were isolated and separately cultivated for 6 days to differentiate into moDC. Gene expression levels were measured in the original monocyte subsets (M) and derived moDC (D). Mean ± SEM; n = 3; Mann–Whitney test for comparison between monocytes and moDC for each gene and subset. *p ≤0.05, **p ≤0.01.
Dulbecco S Modified Eagle S Medium, supplied by Cellgro, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cellgro+dc+medium/medium/10__1016_slash_s0021___9258_ascii40_18_ascii41_35691___6-66-4-8
Average 86 stars, based on 1 article reviews
dulbecco s modified eagle s medium - by Bioz Stars, 2026-10
86/100 stars
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dmem f12  (Cellgro)
86
Cellgro dmem f12
FIGURE 2 | Expression of ANKRD55 and neighboring genes in immature <t>moDC.</t> (A–D) Monocytes were cultivated for 6 days <t>in</t> <t>IL-4/GM-CSF</t> medium for differentiation into immature moDC in the absence (colored curves) or presence (gray curves) of AM-580, and expression levels of the indicated genes were measured by qPCR at the start of the cultivation (day 0) and on days 1, 2, 4, and 6. Mean ± SEM of 3 independent measurements; Friedman test (followed by Dunn’s multiple comparison test) for comparison of data points in each curve with day 0 and Wilcoxon test for comparison of both curves (Control vs. AM580). (E–H) Effect of tolerogenic compounds on gene expression in immature moDC by qPCR. Concentrations of compounds are provided in the Materials and Methods. Mean ± SEM of maximum 5 independent measurements; Friedman test (followed by Dunn’s multiple comparison test). (I) Analysis of ALDH1A2, RARa, and RARg expression by qPCR. Mean ± SEM; n = 6; Friedman test (followed by Dunn’s multiple comparison test). (J) Expression levels of three discrete ANKRD55 splice variants 201, 202, and 204 in immature moDC. Mean ± SEM; n = 10; Wilcoxon test. (K) Classical (Cla; CD14hi/CD16-), intermediate (Int; CD14hi/CD16+), and non- classical (NC; CD14+/CD16+) monocytes were isolated and separately cultivated for 6 days to differentiate into moDC. Gene expression levels were measured in the original monocyte subsets (M) and derived moDC (D). Mean ± SEM; n = 3; Mann–Whitney test for comparison between monocytes and moDC for each gene and subset. *p ≤0.05, **p ≤0.01.
Dmem F12, supplied by Cellgro, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cellgro+dc+medium/dulbecco+s/10__1007_slash_978___1___61779___513___8-6261-26-36
Average 86 stars, based on 1 article reviews
dmem f12 - by Bioz Stars, 2026-10
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d-pbs - 1x  (Welgene inc)
90
Welgene inc d-pbs - 1x
FIGURE 2 | Expression of ANKRD55 and neighboring genes in immature <t>moDC.</t> (A–D) Monocytes were cultivated for 6 days <t>in</t> <t>IL-4/GM-CSF</t> medium for differentiation into immature moDC in the absence (colored curves) or presence (gray curves) of AM-580, and expression levels of the indicated genes were measured by qPCR at the start of the cultivation (day 0) and on days 1, 2, 4, and 6. Mean ± SEM of 3 independent measurements; Friedman test (followed by Dunn’s multiple comparison test) for comparison of data points in each curve with day 0 and Wilcoxon test for comparison of both curves (Control vs. AM580). (E–H) Effect of tolerogenic compounds on gene expression in immature moDC by qPCR. Concentrations of compounds are provided in the Materials and Methods. Mean ± SEM of maximum 5 independent measurements; Friedman test (followed by Dunn’s multiple comparison test). (I) Analysis of ALDH1A2, RARa, and RARg expression by qPCR. Mean ± SEM; n = 6; Friedman test (followed by Dunn’s multiple comparison test). (J) Expression levels of three discrete ANKRD55 splice variants 201, 202, and 204 in immature moDC. Mean ± SEM; n = 10; Wilcoxon test. (K) Classical (Cla; CD14hi/CD16-), intermediate (Int; CD14hi/CD16+), and non- classical (NC; CD14+/CD16+) monocytes were isolated and separately cultivated for 6 days to differentiate into moDC. Gene expression levels were measured in the original monocyte subsets (M) and derived moDC (D). Mean ± SEM; n = 3; Mann–Whitney test for comparison between monocytes and moDC for each gene and subset. *p ≤0.05, **p ≤0.01.
D Pbs 1x, supplied by Welgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cellgro+dc+medium/1%C3%97+dpbs/pm39221957-23-93-111
Average 90 stars, based on 1 article reviews
d-pbs - 1x - by Bioz Stars, 2026-10
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Image Search Results


FIGURE 2 | Expression of ANKRD55 and neighboring genes in immature moDC. (A–D) Monocytes were cultivated for 6 days in IL-4/GM-CSF medium for differentiation into immature moDC in the absence (colored curves) or presence (gray curves) of AM-580, and expression levels of the indicated genes were measured by qPCR at the start of the cultivation (day 0) and on days 1, 2, 4, and 6. Mean ± SEM of 3 independent measurements; Friedman test (followed by Dunn’s multiple comparison test) for comparison of data points in each curve with day 0 and Wilcoxon test for comparison of both curves (Control vs. AM580). (E–H) Effect of tolerogenic compounds on gene expression in immature moDC by qPCR. Concentrations of compounds are provided in the Materials and Methods. Mean ± SEM of maximum 5 independent measurements; Friedman test (followed by Dunn’s multiple comparison test). (I) Analysis of ALDH1A2, RARa, and RARg expression by qPCR. Mean ± SEM; n = 6; Friedman test (followed by Dunn’s multiple comparison test). (J) Expression levels of three discrete ANKRD55 splice variants 201, 202, and 204 in immature moDC. Mean ± SEM; n = 10; Wilcoxon test. (K) Classical (Cla; CD14hi/CD16-), intermediate (Int; CD14hi/CD16+), and non- classical (NC; CD14+/CD16+) monocytes were isolated and separately cultivated for 6 days to differentiate into moDC. Gene expression levels were measured in the original monocyte subsets (M) and derived moDC (D). Mean ± SEM; n = 3; Mann–Whitney test for comparison between monocytes and moDC for each gene and subset. *p ≤0.05, **p ≤0.01.

Journal: Frontiers in immunology

Article Title: Genomic Multiple Sclerosis Risk Variants Modulate the Expression of the ANKRD55 - IL6ST Gene Region in Immature Dendritic Cells.

doi: 10.3389/fimmu.2021.816930

Figure Lengend Snippet: FIGURE 2 | Expression of ANKRD55 and neighboring genes in immature moDC. (A–D) Monocytes were cultivated for 6 days in IL-4/GM-CSF medium for differentiation into immature moDC in the absence (colored curves) or presence (gray curves) of AM-580, and expression levels of the indicated genes were measured by qPCR at the start of the cultivation (day 0) and on days 1, 2, 4, and 6. Mean ± SEM of 3 independent measurements; Friedman test (followed by Dunn’s multiple comparison test) for comparison of data points in each curve with day 0 and Wilcoxon test for comparison of both curves (Control vs. AM580). (E–H) Effect of tolerogenic compounds on gene expression in immature moDC by qPCR. Concentrations of compounds are provided in the Materials and Methods. Mean ± SEM of maximum 5 independent measurements; Friedman test (followed by Dunn’s multiple comparison test). (I) Analysis of ALDH1A2, RARa, and RARg expression by qPCR. Mean ± SEM; n = 6; Friedman test (followed by Dunn’s multiple comparison test). (J) Expression levels of three discrete ANKRD55 splice variants 201, 202, and 204 in immature moDC. Mean ± SEM; n = 10; Wilcoxon test. (K) Classical (Cla; CD14hi/CD16-), intermediate (Int; CD14hi/CD16+), and non- classical (NC; CD14+/CD16+) monocytes were isolated and separately cultivated for 6 days to differentiate into moDC. Gene expression levels were measured in the original monocyte subsets (M) and derived moDC (D). Mean ± SEM; n = 3; Mann–Whitney test for comparison between monocytes and moDC for each gene and subset. *p ≤0.05, **p ≤0.01.

Article Snippet: CD14+ monocytes were isolated from fresh PBMCs by positive selection using CD14 MACS MicroBeads (Miltenyi Biotec, Ref. 130-050-201) and cultured at a density of 106 cells/ml in moDC differentiation medium (containing IL-4/GM-CSF; Miltenyi Biotec, Ref. 130-094-812) for 6 days, with fresh medium added on day 3.

Techniques: Expressing, Comparison, Control, Gene Expression, Isolation, Derivative Assay, MANN-WHITNEY